The game between Laois and Carlow was initially postponed from Wednesday evening to the weekend due to weather conditions.
The latest O'Byrne Cup cancellation leaves Laois without an opponent for their last game in the competition.
Carlow were due to play Billy Sheehan's footballers in Stradbally on Wednesday until the game was postponed due to a waterlogged pitch.
Today Leinster GAA were notified that Carlow were unable to fulfil the fixture due to "Sigerson Cup commitments, injuries and sickness ".
The game will now, not take place.
Laois' first game of their division four league campaign will them making the trip to Markievicz Park to take on Sligo.
Shane Lowry Records Opening Round 64 In UAE
Five Players Renew Their Contracts With Athlone Town
Midlands County's Learn Their O'Byrne Cup Opponents
Clough Ballacolla Aiming To Book Provincial Semi-Final Spot
Shane Lowry In Action At Abu Dhabi Championship.
CCFL: Clonaslee Pick Up Crucial Win Over Mountmellick
Castletown Geoghegan Looking To Make Progress In Leinster
Casey O'Gorman To Race In Final Rounds Of Moto3 Championship
Offaly To Begin Leinster Championship At Home To Dublin
Buccaneers Lose At Home To Navan In AIL
Portlaoise Panthers Keep Up Unbeaten Start In Division One
Leinster Championship Is Wide Open - Chris Conway
Birr Suffer Semi-Final Defeat In Leinster Camogie Championship
Portlaoise Panthers Suffer Defeat In Women's Super League
Portarlington Seal Quarter Final Spot In Leinster Championship
Buccaneers Searching For Back To Back AIL Wins
Leinster Final Place On The Line For Birr
Portarlington Begin Leinster Journey Away To Carlow Champions
Provincial Final The Goal For Milltown