Ciaran Kilduff's side lost to their rivals at the weekend.
Athlone Town's unbeaten run has come to an end at the hands of one of their biggest rivals.
Ciaran Kilduff's side fell to Shelbourne on a final scoreline of 2-1 in the All-Island Cup in Tolka Park on Saturday.
Two goals from Noelle Murray saw her side get the better of Town at the Quarter-final stage, despite an excellent volley from Casey Howe in the first half.
Athlone Town are back in action this weekend, when they take on Shamrock Rovers in the SSE Airtricity Women's Premier Division on Saturday at 4pm in Tallaght Stadium.