Gigginstown say he's been allocated an "unfair weight".
Tiger Roll will not bid for a third Grand National win after connections decided not to run under an "unfair weight".
The 11-year-old became the first horse since Red Rum to secure back-to-back Aintree successes when triumphing in 2018 and 2019.
The latest forfeit stage for the National is today and Gigginstown have confirmed Tiger Roll will be taken out of the race.